Dynamics of Force-Induced DNA Slippage
نویسندگان
چکیده
منابع مشابه
Dynamics of force-induced DNA slippage.
We study the base pairing dynamics of DNA with repetitive sequences where local strand slippage can create, annihilate, and move bulge loops. Using an explicit theoretical model, we find a rich dynamical behavior as a function of an applied shear force f: reptationlike dynamics at f=f(c) with a rupture time tau scaling as N3 with its length N, drift-diffusion dynamics for f(c)<f<f(*), and a dyn...
متن کاملForce-induced DNA slippage.
DNA containing repetitive sequences displays richer dynamics than heterogeneous sequences. In the genome the number of repeat units of repetitive sequences, known as microsatellites, may vary during replication by DNA slippage and their expansion gives rise to serious disorders. We studied the mechanical properties of repetitive DNA using dynamic force spectroscopy and found striking difference...
متن کاملDynamic aspects of DNA DNA-slippage and nucleosome dynamics
xi 1. Properties of DNA 1 1.1. The structure of DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2. Thermodynamics of DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1.2.1. Binding free energies of double stranded DNA . . . . . . . . . . . . 4 1.2.2. Denaturation of long DNA molecules . . . . . . . . . . . . . . . . . 5 1.2.3. DNA melting of homogenous sequences . ....
متن کاملIterative Force-Field Calculation and Molecular Dynamics of Cyclooctanone
Body's iterative force-field computer program has been used to calculate strain energies in cyclooctanone (I). 348 MHZ 1H NMR spectra of (I) have been investigated over the temperature range of 25° to -160°C. Two conformation processes affect the 1H NMR spectrum of (I). Iterative force-field calculations on the conformations and conformational interconversion paths of ...
متن کاملForce regulated dynamics of RPA on a DNA fork
Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Physical Review Letters
سال: 2004
ISSN: 0031-9007,1079-7114
DOI: 10.1103/physrevlett.93.198102